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b lymphocyte cell lines daudi  (ATCC)


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    Structured Review

    ATCC b lymphocyte cell lines daudi
    B Lymphocyte Cell Lines Daudi, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1765 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/daudi+cell+line/Daudi/us12648910-87-1-14
    Average 98 stars, based on 1765 article reviews
    b lymphocyte cell lines daudi - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Amplification:

    Article Title: FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes
    Article Snippet: .. The FCRL3 gene was amplified from cDNA from the Daudi cell line (CCL-213; ATCC) and then cloned into pLVX-IRES-PURO using the SpeI and NotI restriction sites. ..

    Clone Assay:

    Article Title: FCRL3 is an immunoregulatory receptor that restrains the activation of human memory T lymphocytes
    Article Snippet: .. The FCRL3 gene was amplified from cDNA from the Daudi cell line (CCL-213; ATCC) and then cloned into pLVX-IRES-PURO using the SpeI and NotI restriction sites. ..

    Activity Assay:

    Article Title: Methods and compositions for reducing the immunogenicity of chimeric notch receptors
    Article Snippet: Expression of the cognate reporter construct for the synNotch was tested by measuring the constitutive mCherry expression produced from the reporter vector by flow cytometry. .. Cells were assayed for synNotch activity by stimulating the cells for 24 hours by co-culturing with a Daudi cell line expressing high-levels of CD19 antigen (American Type Culture Collection (ATCC) CCL-213TM cells (Daudi cells)) as well as cell lines negative for cell-surface CD19. ..

    Expressing:

    Article Title: Methods and compositions for reducing the immunogenicity of chimeric notch receptors
    Article Snippet: Expression of the cognate reporter construct for the synNotch was tested by measuring the constitutive mCherry expression produced from the reporter vector by flow cytometry. .. Cells were assayed for synNotch activity by stimulating the cells for 24 hours by co-culturing with a Daudi cell line expressing high-levels of CD19 antigen (American Type Culture Collection (ATCC) CCL-213TM cells (Daudi cells)) as well as cell lines negative for cell-surface CD19. ..

    Binding Assay:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: .. Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies.

    Flow Cytometry:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: .. Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies.

    Immunostaining:

    Article Title: Antibody and bispecific antibody targeting LAG-3 and use thereof
    Article Snippet: .. The Daudi cell line (ATCC, CCL-213) was expanded and seeded into 96-well microplate at a density of 2×105 cells/well, and then centrifuged at 1600 rpm for 10 min; thereafter the cells were fixed with 100 μl/well of a fixative solution for immunostaining (Shanghai Beyotime Biotechnology Co., Ltd., Cat. No.: P0098) for half an hour at room temperature. ..

    Titration:

    Article Title: Characterizing CD38 expression in terminally differentiated B cells using variable lymphocyte receptor B tetramers
    Article Snippet: .. Titration of antibodies was performed by serial dilutions of biotinylated VLRB MM3, and VLRB B7 (negative control against SARS-CoV-2 spike protein) on the CD38+ Burkitt’s lymphoma-derived Daudi cell line (ATCC, Manassas, VA, USA). ..

    Negative Control:

    Article Title: Characterizing CD38 expression in terminally differentiated B cells using variable lymphocyte receptor B tetramers
    Article Snippet: .. Titration of antibodies was performed by serial dilutions of biotinylated VLRB MM3, and VLRB B7 (negative control against SARS-CoV-2 spike protein) on the CD38+ Burkitt’s lymphoma-derived Daudi cell line (ATCC, Manassas, VA, USA). ..



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